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quantitative pcr  (Toyobo)


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    Toyobo quantitative pcr
    Quantitative Pcr, supplied by Toyobo, used in various techniques. Bioz Stars score: 96/100, based on 5853 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative pcr/product/Toyobo
    Average 96 stars, based on 5853 article reviews
    quantitative pcr - by Bioz Stars, 2026-03
    96/100 stars

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    PARsylation of TFEB is crucial for its interaction with TCF-1/LEF-1 and Wnt-TFEB target gene expression. (A) TFEB with mutations at residues 237K and 274K is not PARsylated by TNKS1. (B, C) TFEB with mutations in the PARsylation sites does not bind to HA-TCF-1 (B) or HA-LEF-1 (C) in HEK293T cells. (D) The self-interaction of the TFEB mutant, which has mutations at PARsylation sites, was not reduced by the treatment of Wnt3a-CM. HEK293A cells were transfected with TFEB mutants carrying different tags and treated with Wnt3a-CM for 2 hours. (E) Treatment of HEK293A cells with Wnt3a-CM for 2 hours does not reduce the level of oligomer of TFEB PARsylation-defective mutants. (F) PARsylation of TFEB is necessary for Wnt-TFEB target gene expression. HEK293A cells were knocked down and transfected with the indicated constructs, then treated with Wnt3a-CM for 8 hours. The cells were then used for real-time PCR. Statistical analysis was performed using a 2-tailed unpaired t-test, showing exact P- values in each figure. Error bars indicate the standard deviation of triplicate measurements (technical replicates).

    Journal: Molecules and Cells

    Article Title: Tankyrase-1-mediated PARsylation directs TFEB partner switching to regulate selective Wnt target gene expression

    doi: 10.1016/j.mocell.2026.100313

    Figure Lengend Snippet: PARsylation of TFEB is crucial for its interaction with TCF-1/LEF-1 and Wnt-TFEB target gene expression. (A) TFEB with mutations at residues 237K and 274K is not PARsylated by TNKS1. (B, C) TFEB with mutations in the PARsylation sites does not bind to HA-TCF-1 (B) or HA-LEF-1 (C) in HEK293T cells. (D) The self-interaction of the TFEB mutant, which has mutations at PARsylation sites, was not reduced by the treatment of Wnt3a-CM. HEK293A cells were transfected with TFEB mutants carrying different tags and treated with Wnt3a-CM for 2 hours. (E) Treatment of HEK293A cells with Wnt3a-CM for 2 hours does not reduce the level of oligomer of TFEB PARsylation-defective mutants. (F) PARsylation of TFEB is necessary for Wnt-TFEB target gene expression. HEK293A cells were knocked down and transfected with the indicated constructs, then treated with Wnt3a-CM for 8 hours. The cells were then used for real-time PCR. Statistical analysis was performed using a 2-tailed unpaired t-test, showing exact P- values in each figure. Error bars indicate the standard deviation of triplicate measurements (technical replicates).

    Article Snippet: Quantitative real-time PCR (qPCR) was conducted using THUNDERBIRD SYBR qPCR Mix (Toyobo, QPS-201) in accordance with the manufacturer’s instructions.

    Techniques: Targeted Gene Expression, Mutagenesis, Transfection, Construct, Real-time Polymerase Chain Reaction, Standard Deviation